| 摘要 |
<p>A method of automatically sequencing DNA comprises repeatedly determining the next base in the sequence as a function not only of a physical measurement made at that position, but also as a function of previously-determined near by bases in the same sequence. Typically, a computer algorithm is used which predicts the value of the expected measurement at a given position based upon a knowledge of the bases in previous and/or subsequent positions. The predicted measurment is then compared with the actual measurement, and the base chosen at that position is the base which minimises the accumulated error measure for the entire sequence. The preferred algorithm, which may be parallel or sequential, preferably includes physical modelling of the replication effect and of the fluorescence effect.</p> |
