MITOCHONDRIAL COLLECTION AND CONCENTRATION, AND USES THEREOF

摘要

Preparations and methods of producing a concentrated mitochondrial preparation using inverted phase centrifugation are disclosed. The methods include centrifuging a closed- bottom tube containing a heavy phase including a mitochondrial suspension in the portion of the tube proximal to the axis of rotation and a light phase in the portion of the tube distal to the axis of rotation. The centrifugation causes the suspension to form a concentrated mitochondrial layer at the interface between the heavy layer and the light layer, and the fluid at this interface is collected to produce the concentrated mitochondrial preparation. The concentrated mitochondrial preparation can be used for various applications, including in vitro fertilization applications.