Single-Walled Carbon Nanotube Biosensor for Detection of Glucose, Lactate, and Urea

摘要

A single-walled carbon nanotube-based micron scale multiplex biosensor is provided that enables the detection of glucose, lactate, and urea. The sensor is based on modification of semiconducting single-walled carbon nanotubes using a linker that non-covalently associates with the nanotubes and covalently couples to an enzyme. Reaction of a physiological substrate with the enzyme results in increased resistance of the nanotubes within the sensor. The sensor is suitable for use in patient monitoring, particularly in a clinical setting. Incorporation of read out electronics and an RF signal generator into the sensor device enables it to communicate to a relay station or remote receiver. Methods are also provided for fabricating the biosensor device and using the device for detection.

主权项

1. A microscale biosensor for detecting a chemical agent, the biosensor comprising:
a substrate; a conductive layer attached to a surface of the substrate, the conductive layer forming at least one pair of electrodes with an insulating gap between the electrodes; and a conductive bridge consisting essentially of one or more functionalized single-walled carbon nanotubes contacting the electrodes and bridging the gap between the electrodes; wherein the one or more nanotubes are functionalized via a linker with an enzyme that reacts with said chemical agent, whereby the conductivity of the conductive bridge is modified; and wherein the linker is 1-pyrenebutanoic acid succinimidyl ester.