COMPOSITIONS AND METHODS FOR DETECTING HEV NUCLEIC ACID

摘要

Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

主权项

1. A combination of at least two oligomers for determining the presence or absence of hepatitis E virus (HEV) in a sample, said oligomer combination comprising:
at least two amplification oligomers for amplifying a target region of an HEV target nucleic acid, wherein
(a) at least one amplification oligomer is selected from the group consisting of
(i) an oligomer comprising a target-hybridizing sequence that is from about 14 to about 23 contiguous nucleotides contained in the sequence of SEQ ID NO:63 and that includes at least the sequence of SEQ ID NO:26, including RNA equivalents and DNA/RNA chimerics thereof, and(ii) an oligomer comprising a target-hybridizing sequence that is from about 14 to about 23 contiguous nucleotides contained in the sequence of SEQ ID NO:16, including RNA equivalents and DNA/RNA chimerics thereof; and(b) at least one amplification oligomer comprises a target-hybridizing sequence that is from about 17 to about 28 contiguous nucleotides contained in the sequence of SEQ ID NO:47 and that includes at least the sequence of SEQ ID NO:25, including RNA equivalents and DNA/RNA chimerics thereof.