发明名称 |
Method for rendering ligase-based amplification products unamplifiable |
摘要 |
Methods for sterilization of ligase-based amplification products, including products of the Ligation Amplification Reaction (LAR), involving treatment of nucleic acid after amplification to render such nucleic acid incapable of serving as a template for further amplification. Both random and site-specific addition of sterilizing compounds, and more specifically, photoactivation compounds, render amplification product subsequently unamplifiable. Self-sterilizing primers are also employed allowing for a homogenous detection of ligase-based amplification product.
|
申请公布号 |
US5532146(A) |
申请公布日期 |
1996.07.02 |
申请号 |
US19940263020 |
申请日期 |
1994.06.20 |
申请人 |
HRI RESEARCH, INC. |
发明人 |
CIMINO, GEORGE D.;ISAACS, STEPHEN T.;TESSMAN, JOHN W. |
分类号 |
B01L7/00;C07D311/18;C07D493/04;C07D519/00;C07H21/00;C12N15/10;C12P19/34;C12Q1/68;C12Q1/70;(IPC1-7):C12P19/34 |
主分类号 |
B01L7/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|