| 摘要 |
Resveratrol is naturally occurring polyphenol having various properties, such as anti-inflammatory, anti-oxidant and neuroprotection effects. According to the present invention, the effect of resveratrol upon treatment of Type 1 fibroblasts derived from a patient suffering from Gaucher disease is evaluated. The effect of resveratrol upon cell viability is evaluated by MTT analysis. To determine the effect of resveratrol upon the cells of a patient suffering from Gaucher disease, Western immunoblot is carried out to evaluate variations in expression of AIF, Bax, cleaved caspase-3, ACAT1, E3BP and CS. In addition, thin film chromatography is carried out to determine the intracellular glucosylceramide (glucocerebroside) concentration in the cells treated with resveratrol. As a result, the cells treated with resveratrol shows a significant increase in viability as compared to the control cells. After the treatment with resveratrol, the expression levels of cell apoptosis factors, AIF, Bax and cleaved caspase-3 are increased in a dose-dependent manner. The TLC results demonstrate a significant decrease in glucosylceramide in the cells of a patient suffering from Gaucher disease, treated with resveratrol. Such results demonstrate that resveratrol decreases cell apoptosis, reduces glucosylceramide concentration, and thus can be used as a treatment agent. |
