发明名称 USE OF SPERMIDINE TO RELIEVE INHIBITION OF LIGASE USE OF SPERMIDINE TO RELIEVE INHIBITION OF LIGASE CHAIN REACTION IN A CLINICAL TEST SAMPLE CHAIN REACTION IN A CLINICAL TEST SAMPLE
摘要 An improved method of amplifying target nucleic acids and particularly, to a method of alleviating the inhibitory effect of certain samples on ligase chain reaction amplifications. The relief of inhibition is accomplished by adding to the amplification mixture a polyamine reagent such as spermidine, in effective amounts, typically 1 to 4 nM. In other embodiments, the presence of spermidine allows for amplification to proceed in the presence of reduced concentrations of MgCl2 and ligase.
申请公布号 WO9612824(A3) 申请公布日期 1996.06.27
申请号 WO1995US12874 申请日期 1995.10.18
申请人 ABBOTT LABORATORIES 发明人 DAVIS, ALAN, H.;CAO, JIANLI;LEE, EUI, H.
分类号 C12Q1/68 主分类号 C12Q1/68
代理机构 代理人
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