发明名称 |
RECOMBINANT DNA MOLECULES AND EXPRESSION VECTORS FOR ERYTHROPOIETIN |
摘要 |
A recombinant DNA molecule adapted for transfection of a host cell comprising a nucleic acid molecule encoding mammalian erythropoietin, an expression control sequence operatively linked thereto and at least one SAR element. The invention also relates to expression vectors having the recombinant DNA molecule and to mammalian cells transformed with the expression vector. The mammalian cells lack multiple copies of an amplified amplification gene and are capable of expressing recombinant EPO in vitro at levels of at least 1,500 u/10<6> cells in 24 hours. The invention further relates to a method of expressing recombinant mammalian erythropoietin using the expression vectors and to a transgenic non-human animal or embryo whose germ cells and somatic cells contain a DNA construct having the recombinant DNA molecule of the invention.
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申请公布号 |
WO9619573(A1) |
申请公布日期 |
1996.06.27 |
申请号 |
WO1995CA00696 |
申请日期 |
1995.12.18 |
申请人 |
CANGENE CORPORATION;DELCUVE, GENEVIEVE |
发明人 |
DELCUVE, GENEVIEVE |
分类号 |
C07K14/505;C12N15/12;C12N15/67;C12N15/85;(IPC1-7):C12N15/12;C12N5/10;A01K67/027 |
主分类号 |
C07K14/505 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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