发明名称 |
METHODS AND MATERIALS FOR THE IMMUNOASSAY OF APOLIPOPROTEIN(a) AND LIPOPROTEIN(a) |
摘要 |
The present invention relates to monoclonal antibodies and a method for the quantification of apolipoprotein(a) and lipoprotein(a) present in human body fluids. The method is not sensitive to the presence of plasminogen or the apo(a) kringle 4 repeats. The assay involves the addition of the test sample to an immobilized anti-apo(a) antibody and forming a first immobilized complex of apolipoprotein(a)/anti-apo(a) first antibody, contacting first immune complex with an anti-apo(a) monoclonal antibody specific for apo(a) with non crossreactivity to plasminogen and which does not identify the kringle 4 type 2 apo(a) repeats and thereafter quantitating apo(a) based on the amount of bound anti-apo(a) monoclonal antibody. Immobilized goat or rabbit anti-human apo(a) antibody can be employed as the first capture antibody and the mouse anti-apo(a) monoclonal antibody as second antibody. The amount of anti-apo(a) monoclonal antibody bound is quantitated through binding of a third antibody enzyme conjugant, e.g., goat anti-mouse antibody/horseradish peroxidase (HRP) conjugate, followed by reaction with a suitable substrate such as o-phenylenediamine dihydrochloride.
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申请公布号 |
WO9619500(A1) |
申请公布日期 |
1996.06.27 |
申请号 |
WO1995US16613 |
申请日期 |
1995.12.18 |
申请人 |
UNIVERSITY OF WASHINGTON |
发明人 |
MARCOVINA, SANTICA, M.;ALBERS, JOHN, J. |
分类号 |
C07K16/18;G01N33/92;(IPC1-7):C07K16/00;C07K16/44;C12N5/12;G01N33/53;G01N33/577 |
主分类号 |
C07K16/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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