| 摘要 |
<p>A pancreas-derived factor has been identified which lowers serum calcium and inhibits bone resorption, and also stimulates proliferation of MG-63 osteoblast-like cells. The factor has been purified to homogeneity from porcine pancreas and obtained definitive amino acid sequence has been obtained (SEQ ID NO:1). This factor bears homology to a human pancreatic elastase, elastase IIIB. Experiments with recombinant human pancreatic elastase IIIB as well as neutralizing antibodies to human pancreatic elastase IIIB demonstrate that the biological effects of native PX are mimicked by those of recombinant human elastase IIIB both in vitro and in vivo. Numerous diagnostic and therapeutic applications are contemplated, including drug screening assays, test kits for diagnosing and monitoring conditions characterized by changes in bone cell population, and compositions and methods for treating undesirable bone resorption or accretion.</p> |
