发明名称 |
Penicillin V amidohydrolase gene from fusarium oxysporum |
摘要 |
<p>The following sequences are new: (a) a PVA DNA or cDNA (I) sequence encoding the 507 or 532 amino acid sequences given in the specification; (b) the 240 bp PVA gene promoter (II) given in the specification; (c) 23-33 bp long PVA gene fragments and encoded peptides; (d) a sequence complementary to that of (a); (e) a sequence capable of hybridising under stringent conditions to that of (d); (f) a degenerate oligonucleotide probe and sequences used in the construction of the probe; and (g) mutagenic oligonucleotides used in vector construction. Also claimed are (1) a polypeptide encoded by (I); (2) expression vectors comprising (I) and promoter (II); (3) a host cell contg.an expression vector of (2); (4) N-terminal peptides of six isolated major PVA fragments (A, B1, B2, C, D and E) and (5) biologically pure samples of Escherichia coli ATCC 69720, 69721 and 69772.</p> |
申请公布号 |
EP0718402(A2) |
申请公布日期 |
1996.06.26 |
申请号 |
EP19950120437 |
申请日期 |
1995.12.22 |
申请人 |
BRISTOL-MYERS SQUIBB COMPANY |
发明人 |
CHIANG, SHU-JEN;BURNETT, WILLIAM V. JR.;TONZI, SEAN M. |
分类号 |
C12N1/15;C12N15/55;C07H21/04;C07K1/22;C07K14/195;C07K14/37;C07K14/41;C12N1/21;C12N9/06;C12N9/84;C12N9/86;C12N15/09;C12N15/11;C12N15/63;C12N15/80;C12P21/02;C12R1/19;C12R1/77;(IPC1-7):C12N15/55 |
主分类号 |
C12N1/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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