| 摘要 |
<p>The present invention relates to a process for producing a human matrilysin characterized in that a human promatrilysin is expressed in E. coli and secreted into the periplasm thereof; the inclusion body is formed, the human promatrilysin is solubilized with a urea solution, purified and renatured to obtain the active enzyme (active-type matrilysin). By this process, the active human matrilysin can be easily produced from transformed E. coli.</p> |
