发明名称 |
Method for the purification of PC protein from Borrelia burgdorferi +B |
摘要 |
A method of purifying B. burgdorferi proteins comprising the steps of (a) disrupting a B. burgdorferi cell and fractionating the disrupted cell into membrane and cytoplasmic components; (b) resuspending the membrane component in a non-denaturing detergent thereby producing a solubilized protein and an insolubilized material and then separating said solubilized protein from said insolubilized material; (c) subjecting the solubilized protein to ion-exchange chromatography so as to produce protein fractions; and (d) assaying the protein fractions to identify those fractions which contain proteins of interest wherein the purified proteins of interest are in a biologically active form suitable for use in vaccines, is disclosed.
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申请公布号 |
US5530103(A) |
申请公布日期 |
1996.06.25 |
申请号 |
US19930031295 |
申请日期 |
1993.03.12 |
申请人 |
IMMUNO AKTIENGESELLSCHAFT |
发明人 |
LIVEY, IAN;DORNER, FRIEDRICH |
分类号 |
A61K39/00;A61K39/02;A61P31/04;C07K14/20;C12N15/31;C12P21/02;G01N33/569;G01N33/68;(IPC1-7):C07K1/16;C07K1/18;C07K1/22 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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