发明名称 |
PRODUCTION OF PEPTIDES USING RECOMBINANT FUSION PROTEIN CONSTRUCTS |
摘要 |
A method for the isolation and/or purification of a recombinant peptide by employing a fusion protein construct which includes a carbonic anhydrase and a variable fused polypeptide is provided. The method includes precipitating either the fusion protein construct or a fragment of the fusion protein construct including the carbonic anhydrase. Inclusion bodies which includes the fusion protein construct and a method of producing a peptide which includes expressing the fusion protein construct as a part of an inclusion body are also provided. Fusion protein constructs which include a carbonic anhydrase and certain target peptides are also provided.
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申请公布号 |
CA2206848(A1) |
申请公布日期 |
1996.06.13 |
申请号 |
CA19952206848 |
申请日期 |
1995.12.07 |
申请人 |
BIONEBRASKA, INC. |
发明人 |
HOLMQUIST, BARTON;HENRIKSEN, DENNIS B.;WAGNER, FRED W.;DE LA MOTTE, REBECCA S.;MANNING, SHANE D.;PARTRIDGE, BRUCE E.;STOUT, JAY S. |
分类号 |
C12N15/09;C07K1/113;C07K1/30;C07K5/103;C07K5/113;C07K7/06;C07K14/60;C07K14/605;C07K14/635;C07K19/00;C12N1/21;C12N9/88;C12P21/02;C12R1/19;(IPC1-7):C12N9/96;C12P21/06;C07K1/22 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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