摘要 |
<p>A method for the isolation and/or purification of a recombinant peptide by employing a fusion protein construct which includes a carbonic anhydrase and a variable fused polypeptide is provided. The method includes precipitating either the fusion protein construct or a fragment of the fusion protein construct including the carbonic anhydrase. Inclusion bodies which includes the fusion protein construct and a method of producing a peptide which includes expressing the fusion protein construct as a part of an inclusion body are also provided. Fusion protein constructs which include a carbonic anhydrase and certain target peptides are also provided.</p> |