| 摘要 |
The present invention relates to a method for the amplification of a target nucleotide sequence using a first primer comprising 1) a target binding nucleotide moiety which is substantially complementary to the desired portion of the target nucleotide sequence; and 2) a polynucleotide tail. The first primer is such that while an extension product thereof may itself serve as a template for primer extension to form an amplification primer extension product, formation of an amplification primer extension product comprising a sequence complementary to the polynucleotide tail is inhibited.
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