CRYOPRESERVATION OF CULTURED EPITHELIAL SHEETS

摘要

Disclosed is a method of cryopreserving a sheet of living, cultured epthelial cells for use as a skin wound dressing. The method ensures that the integrity of the sheet will be maintained, and it preserves a significant number of cells in mitotically competent, physiologically healthy form. It involves use of a non cell penetrating cryoprotectant and certain freezing and thawing conditions.