Assay for Ribozyme target site

摘要

Method for determining target site accessibility for a ribozyme by providing a DNA oligonucleotide having a nucleotide sequence complementary to the RNA target. The DNA oligonucleotide is contacted with the RNA target in the presence of an agent, such as RNAseH, such that the RNAseH cleaves the RNA target when the DNA and RNA form a duplex. The occurrence of cleavage is detected by any standard methodology. Those DNA oligonucleotides which provide a detectable level of cleavage indicate that the RNA target site is accessible to ribozyme.