| 摘要 |
This invention is directed to novel 3,4-disubsti-tuted and 3,4,6-trisubstituted pyrazolo¢3,4-d!pyrimidines and to the use of these compounds in the preparation of oligonucleotides. The invention is also directed to nucleosides and mono- and oligonucleotides comprising at least one of these pyrazolopyrimidines, and to the use of the resulting novel oligonucleotides for diagnostic purposes. The pyrazolopyrimidines of the present invention are of the formula (I): (I) <IMG> The substituents are defined in the disclosure. The novel oligo- and polynucleotides are useful in the identification, isolation, localization and/or detection of complementary nucleic acid sequences of interest in cell-free or cellular systems. The invention further provides a method for identifying target nucleic acid sequences that comprises utilizing an oligo- or polynucleotide probe comprising at least one labeled pyrazolo¢3,4-d!pyrimidine of the present invention.
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