METHOD OF PREPARING, CLONING AND EXPRESSION OF GENE, GENES, IMMUNOTOXINS, GENE PRODUCTS, DNA, CELL, METHOD OF GENE VERIFICATION AND A METHOD OF MUTATION CORRECTION

摘要

FIELD: molecular biology. SUBSTANCE: invention finds nucleotide sequence of synthetic gene for plant toxin gelonine and a method of preparing, cloning and expression of this synthetic gene. DNA sequence for gelonine synthetic gene is given as sequence ID N 1. Invention proposes also expression vectors containing DNA sequences for gelonine and cells transformed with these vectors. Also, immunotoxin containing antibodies conjugated with protein gelonine is proposed. EFFECT: improved method of procedures.