| 摘要 |
<p>The invention relates to an expression vector for the transcomplementation of a retroviral vector which provides for the transfer and/or integration within the genome of a target cell, of a selected nucleotide sequence (called 'transgenic sequence'), characterized in that it comprises; a) a nucleotide sequence called sequence env, coding for a polypeptide derived from the envelope (polypeptide ENV) of a retrovirus of the spumavirus family, the polypeptide ENV providing for the encapsidation of retroviral RNA, b) transcription regulating signals controlling the expression of the sequence env. The invention also relates to a eucaryotic cell for the encapsidation by transcomplementation of recombinant retroviral RNA providing for the transfer and/or integration within the genome of a target cell, of a selected nucleotide sequence (transgenic sequence) recombined with the above disclosed vector.</p> |
