PCR AMPLIFICATION OF REARRANGED GENOMIC VARIABLE REGIONS OF IMMUNOGLOBULIN GENES

摘要

The invention features methods for isolating immunoglobulin variable region genes, and the use of these genes in the production of chimeric and isotype switched antibodies. The invention also features substantially pure DNA encoding a variable region of the antibody produced by hybridoma cell line HNK-20, and chimeric antibodies containing this variable region.