发明名称 |
PLASMIDS SUITABLE FOR IL-2 EXPRESSION |
摘要 |
The present invention relates to plasmids suitable for IL-2 expresion, particularly, human IL-2 expression, and related methods. The plasmid consists essentially of an expression facilitating sequence derived from the immediate-early promoter region of CMV, an expression facilitating sequence derived from the transcriptional termination/polyadenylation signal sequence of the BGH gene, a sequence coding for the eukaryotic expression of an IL-2, possessing a bioactivity of the complete IL-2, operably linked to both of said expression facilitating sequences. The sequence coding for the expression of an IL-2 is a sequence encoding a mature IL-2 and a non-IL-2 leader peptide that augments eukaryotic expression compared to a wild-type IL-2 leader peptide. Optionally, the plasmid also includes a non-mammalian origin of replication and a sequence operably encoding a selectable marker.
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申请公布号 |
WO9617063(A1) |
申请公布日期 |
1996.06.06 |
申请号 |
WO1995US15020 |
申请日期 |
1995.11.28 |
申请人 |
VICAL INCORPORATED |
发明人 |
HOBART, PETER, M.;MARGALITH, MICHAL;PARKER, SUEZANNE, E.;KHATIBI, SHIRIN |
分类号 |
C12N15/09;A61K31/70;A61K35/76;A61K38/00;A61K38/20;A61K47/44;A61K48/00;A61P35/00;C07K14/55;C12N5/08;C12N15/26;C12N15/62;C12N15/85;C12P21/02;C12P21/04;(IPC1-7):C12N15/26 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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