DNA CODING FOR PROTEIN OF LIGAND-BONDING REGION CONTAINING CRH REGION OF GRANULOCYTE COLONY STIMULATING FACTOR RECEPTOR

摘要

PURPOSE: To obtain the subject new DNA coding for a protein of a ligand- bonding region containing a region complementary to a cytokinin receptor of a granulocyte colony stimulating factor (G-CSF) receptor and giving a protein useful e.g. for the treatment of G-CSF-dependent diseases. CONSTITUTION: This new DNA codes for a protein of a ligand-bonding region containing a region complementary to a cytokinin receptor of a granulocyte colony stimulating factor(G-CSF) receptor having an amino acid sequence containing the amino acid sequence of the formula and useful e.g. for the production of a G-CSF receptor protein useful for the research of diseases relating to the mutual action of G-CSF receptor and a ligand and the treatment and prevention of G-CSF-dependent diseases or abnormalities such as leukemia caused by the abnormal proliferation of granulocyte. The DNA can be produced from a plasmid containing the gene of human G-CSF receptor by treating the plasmid with restriction enzymes and cloning by PCR method using the N- terminal and C-terminal primer of the amino acid sequence containing the ligand-bonding region.