| 摘要 |
New chimeric protein (A) with fibrinolytic and thrombin-inhibiting properties comprises a plasminogen-activating sequence (PAS) coupled at its C terminus to sequence (B): Ser-X1-X2-X3-X4-X5-Pro-Arg-Pro-Y1-Y2-Y3-Asn-Pro-Z (B) X1 = Pro or Leu; X2 = Gly, Val or Pro; X3 = Lys, Val, Arg, Gly or Glu; X4 = Ala, Val, Gly, Leu, or Ile; X5 = Gly, Phe, Trp, Tyr or Val; Y1 = Phe, Tyr or Trp; Y2 = Leu, Ala, Gly, Ile, Ser or Met; Y3 = Leu, Ala, Gly, Ile, Ser or Met; Y4 = Arg, Lys or His; Z = Gly-Asp-Z1-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gln; Z1 = Phe, Tyr, (C), (D) or (E) Asn-Asp-Lys-Tyr-Glu-Pro-Phe-Glu-Glu-Tyr-Leu-Gln (C); Ser-Asp-Phe-Glu-Glu-Phe-Ser-Leu-Asp-Asp-Ile-Glu-Gln (D); Ser-Glu-Phe-Glu-Glu-Phe-Glu-Ile-Asp-Glu-Glu-Glu-Lys (E). Also new are plasmids for prodn. of (A) in E. coli in which the operon contains a regulatable promoter, Shine-Dalgarno sequence (ribosome binding site), start codon, synthetic gene for (A) and 1 or 2 downstream terminators. |
