| 摘要 |
The present invention is directed to a DNA encoding a biologically active fragment of a thermostable, full length DNA polymerase I enzyme of Thermus flavus. More particularly, the invention is directed to a DNA encoding an approximately 63,000 dalton DNA polymerase that lacks 274 amino acids from the N-terminus of the approximately 94,000 dalton T. flavus DNA polymerase I, and to the protein encoded thereby which has been designated the T. flavus DNA polymerase I exo fragment. The enzyme fragments are useful in DNA sequencing, Thermal Cycle Labeling, Polymerase Chain Reaction, and other molecular biological applications. |
