| 摘要 |
PURPOSE:To easily measure the concn. of the substrate in a biosample and to improve measurement accuracy by printing an electrode system on an insulating substrate and forming an enzyme layer consisting of an oxidation-reduction enzyme and hydrophilic high polymer and an electron receptive layer thereon. CONSTITUTION:The electrode system consisting of a counter electrode 2, a measuring electrode 3 and a reference electrode 4 is formed by screen printing of conductive carbon paste on the insulating substrate 1 and drying the paste by heating. An insulating layer 5 is formed partially thereon by similar printing and heating and the respective electrode parts 2 to 4 are made to remain so as to act as electrochemical effect parts. Further, a CMC (carboxymethyl cellulose)-GOD (glucose oxidase) layer 6 which is the enzyme layer consisting of the hydrophilic high polymer and the oxidation-reduction enzyme is further provided on the surface of the parts 2 to 4. Miniaturization is enabled and the reaction is expedited by the formation of the two independent layers in proximity to each other. The wettability of the electrode surface is improved by the hydrophilic high polymer and the measurement with the good accuracy is enabled. |
