| 摘要 |
PURPOSE: To obtain the subject ribozyme for preventing and treating AIDS and tumors, etc., having high cleavage activity, specifically cutting polynucleotide, RNA, etc., comprising two polyribonucleotide containing a specific nucleotide sequence. CONSTITUTION: This new domain conversion hairpin type ribozyme comprises two polyribonucleotide containing a nucleotide sequence of the formula (V is a uracilnucleotide; C is a cytosine nucleotide; A is an adenine nucleotide; G is a guanine nucleotide; B1 to Bh , D1 to De and E1 to Ej are each either of V, C, A and G; F1 to Fj are each a nucleotide complementary with E1 to Ej ; S is A or C; Q1 to Qn , W1 -Wh and X1 to Xm are each either of V, C, A or G; R1 to Rn are each a nucleotide complementary with Q1 to Qn ; (h) is an integer of 1-50; (e), (j), (k), (m) and (n) are each an integer of 1-10), has higher cleavage activity, specifically cuts polynucleotide or RNA in organism efficiently and is effective for preventing and treating diseases such as AIDS and various tumors derives from RNA. |
