发明名称 |
Secretion sequence for the production of a heterologous protein in yeast |
摘要 |
The invention generally features a novel recombinant DNA molecule useful for producing a heterologous protein, e.g., insulin-like growth factor-I, in yeast. The DNA molecule includes the following sequences joined in frame and in the following 5' to 3' progression: a sequence encoding a signal peptide of a yeast secreted protein; a sequence encoding at least 5 amino acids of the N-terminal region of a mature acid phosphatase protein, a sequence encoding a heterologous protein. Preferably the DNA molecule also encodes a spacer element that includes a proteolytic cleavage site.
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申请公布号 |
US5521086(A) |
申请公布日期 |
1996.05.28 |
申请号 |
US19930122889 |
申请日期 |
1993.09.16 |
申请人 |
CEPHALON, INC. |
发明人 |
SCOTT, RICHARD W.;BRIERLEY, RUSSELL A.;HOWLAND, DAVID S. |
分类号 |
C12N15/09;C07K14/39;C07K14/65;C07K19/00;C12N1/11;C12N1/19;C12N9/16;C12N15/81;C12P21/02;C12R1/645;(IPC1-7):C12N1/19;C12N1/21;C12N15/62 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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