| 摘要 |
A method of fractionating components separated in liquid chromatography. The method comprises the steps of applying a sample to a column filled with a separation media while supplying an eluent to the column, causing eluent portions containing components in the sample to flow into a fractionation tube connected to the end of an eluent flow path, holding the eluent portions in the fractionation tube, and injecting air into a rear portion of the eluent portions in the fractionation tube.
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