| 摘要 |
<p>PURPOSE: To enable high-sensitivity determination of human tissue inhibitor of metalloproteinase-1(TIMP-1) which is useful in diagnosis of pulmonary fibrosis or the like by using a labeled monoclonal antibody against the TIMP-1. CONSTITUTION: TIMP-1 in a specimen is determined in high sensitivity through a sandwich enzyme immunoassay technique by using a labeled anti-human TIMP-1 mouse clonal antibody which reacts with human TIMP-1, but not with human TIMP-2 and bovine TIMP-1 under a reduced and a non-reduced conditions in the immunoblotting after SDS electrophoresis of human TIMP-1. This antibody is an IgG which does not inhibit the collagenase activity of the TIMP-1, and, when combining the labeled antibody with another antibody immobilized to an immunoplate prepared by immobilizing an anti-human TIMP-1 mouse clonal antibody having its reactive site different from that of the above antibody, the human TIMP-1 in a specimen can determined in high sensitivity.</p> |
