| 摘要 |
<p>PURPOSE: To provide a method for obtaining cells for producing a polysaccharide, capable of producing a polysaccharide in much higher productivity, by improving a conventional method for manufacturing polysaccharide producing cells, by subjecting a callus of plant cells extracellularly secreting a polysaccharide to subculture. CONSTITUTION: In this method for manufacturing polysaccharide producing cells by subjecting a callus of plant cells extracellularly secreting a polysaccharide to subculture, the subculture is carried out in a liquid medium containing a metal salt in a high concentration in a range of 30-200mM to provide the objective method for producing polysaccharide producing cells. Polysaccharide producing cells extracellularly secreting a polysaccharide having improved polysaccharide productivity and stress resistance are obtained by subculture in a callus state in a liquid medium containing the metal salt in a high concentration.</p> |
