IMPROVED METHOD OF DIFFERENTIATION OF VACCINE AND PATHOGEN STRAINS OF CATTLE BRUCELLI

摘要

FIELD: microbiology, molecular biology. SUBSTANCE: DNA of brucelli contains the unique repeated nucleotide sequences showing complementarity with that of synthetic oligonucleotide /CAC/.. Hypervariable sequences can be detected by digestion of brucella DNA with restriction enzyme NcoI followed by elecetrophoresis and hybridization with DNA-probe /CAC/.. Bands pattern (sites of unique nucleotide sequence) are specific for different brucella strains that ensures to differentiate thereof. EFFECT: increased safety and specificity of method for identification and differentiation of brucelli.