| 摘要 |
The invention relates to modified cellulose-binding domains (CBD), and more particularly to biotinylated CBDs that show a binding affinity to cellulose similar to unmodified CBDs. Biotinylation of the CBD allows for efficient binding of biotin-binding molecules, e.g. avidin or streptavidin, to cellulose and the resultant matrix is appropriate for use as a universal affinity system. In addition, complexes of avidin or streptavidin and the biotinylated CBDs of the invention, through interaction with additional biotinylated component(s), may be used in affinity chromatography columns, diagnostic kits, enzyme reactors, drug and chemical delivery systems, and many other applications known for the avidin-biotin system in various fields of biology, biochemistry, and medicine. The invention further relates to a process for overexpression of the soluble form of CBD of the scaffoldin subunit from the cellulosome of Clostridium thermocellum in suitable host cells. |
