METHOD OF DETERMINING CHLORIDE ION

摘要

A method of determining chloride ions contained in a specimen in an aqueous medium by using an alpha -amylase deactivated by a chelating agent, which comprises adding adenosine triphosphate and an enzyme having a glucokinase activity to a specimen to eliminate glucose contained therein, deactivating the enzyme, and measuring the quantity of glucose formed by the reaction of an alpha -amylase activated by chloride ions by using an oligosaccharide as the substrate. This method is useful as a clinical examination method, is not affected by glucose and maltose also present in the specimen, and has a high accuracy.