发明名称 |
High level recombinant protein production using conditional helper-free adenovirus vector |
摘要 |
The present invention relates to methods and compositions involving an improved recombinant transfer vector for introducing a DNA sequence, encoding a recombinant protein, into an adenovirus genome in generating recombinant adenovirus. Recombinant protein production, in cells infected with the recombinant adenovirus, can approach levels as high as 15-20% of total cellular proteins. The improved transfer vector includes an expression cassette comprising sequentially a transcription promoter, a high efficiency leader, at least one splicing signal, an enhancer-like sequence, a cloning site and a plurality of polyadenylation sites.
|
申请公布号 |
US5518913(A) |
申请公布日期 |
1996.05.21 |
申请号 |
US19940232998 |
申请日期 |
1994.04.22 |
申请人 |
NATIONAL RESEARCH COUNCIL OF CANADA |
发明人 |
MASSIE, BERNARD;LANGELIER, YVES;LAMARCHE, NATALIE |
分类号 |
C12N7/01;C12N15/861;C12N15/866;(IPC1-7):C12N7/01;C12N15/85 |
主分类号 |
C12N7/01 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|