| 摘要 |
A color-forming coupler is provided which can be used in test compositions, as well as methods and apparatus for detection of hydrogen peroxide. The color-forming coupler compounds react with a hydrazone or an aminoantypyrine in the presence of peroxidase and hydrogen peroxide to produce a darkly colored compound. In the presence of peroxidase and hydrogen peroxide, the couplers of the present invention react with a hydrazone or an aminoantipyrine to produce a deeply colored dye, which can be used to give a visual indication of the analyte present in the sample. These couplers can be used in diagnostic tests in which the analyte is converted to hydrogen peroxide. The color-forming couplers are N,N-disubstituted anilines of the formula shown in FIG. 1 , wherein R1 and/or R2=H, C1-C9 alkyl, C1-C9-C9 alkoxy, NR3R4 (where R3 and/or R4=H, C1-C9 alkyl, aryl, or heteroryl), F, Cl, Br, I, COOR5 (where R5=H, C1-C9 alkyl, aryl, or heteroaryl), CN, CONR6R7 (where R6 and/or R7=H, C1-C9 alkyl, aryl, or heteraryl), aryl, aryloxy, heteroaryl, heteroaryloxy; and R11, R12, R13, and/or R14=H, C1-C9 alkyl, C1-C9 alkoxy, or NR15R16 wherein at least one of R11 and R14 is NR15R16 wherein only one of R11 and R14 is H or C1-C2 alkyl; where R15 and/or R16=H, C1-C9 alkyl, aryl, or heteroaryl, F, Cl, Br, I, COOR17, where R17=H, C1-C9 alkyl, aryl, or heteroaryl, CN, CONR18R19 (where R18 and/or R19=H, C1-C9 alkyl, aryl, or heteroaryl, aryl, aryloxy, heteroaryl, heteroaryloxy; and n=0-10 and m=0-10 and Z and/or y=H, OH, SH, COOR8 (where R8=H, C1-C9 alkyl, aryl, or heteroaryl), CN, NR9R10 (where R9 and/or R10=H, C1-C9 alkyl, aryl, or heteroaryl), NR20NHR21 (where R20 and/or R21=H, C1-C9 alkyl, aryl, or heteroaryl), where at least one of Z or Y is COOR8, CN NR9R10, or NR20NHR21 or 4-amino antipyrines.
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