| 摘要 |
Methods are described for the isolation, characterization, and expression of flea allergens. cDNA clones encoding three major Ctenocephalides felis felis allergens are provided (pCten f I, pCten f II, pCten f III). The clones contain coding regions, and structural features involved in the transcription and translation of the flea allergens. The proteins encoded by the cDNAs have molecular weights of approximately 15,000, 16,000, and 11,000 daltons respectively. The recombinant proteins are shown to retain the antigenicity of the native molecule.
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