发明名称 |
CLONING AND EXPRESSION OF CDNA FOR HUMAN DIHYDROPYRIMIDINE DEHYDROGENASE |
摘要 |
The invention relates to methods and compositions that are useful for detecting deficiencies in dihydropyrimidine dehydrogenase (DPD) levels in mammals including humans. Cancer patients having a DPD deficiency are at risk of a severe toxic reaction to the commonly used anticancer agent 5-fluorouracil (5-FU). Claimed are DPD genes from human and pig, methods for detecting the level of nucleic acids that encode DPD in a patient, and nucleic acids that are useful as probes for this purpose. Also claimed are methods for expressing DPD in heterologous organisms. Expression vectors that employ a DPD nucleic acid as a selectable marker are also claimed. This selectable marker functions in both prokaryotes and eukaryotes. |
申请公布号 |
WO9608568(A3) |
申请公布日期 |
1996.05.17 |
申请号 |
WO1995US12016 |
申请日期 |
1995.09.07 |
申请人 |
THE GOVERNMENT OF THE UNITED STATES OF AMERICA, RE |
发明人 |
GONZALEZ, FRANK, J.;FERNANDEZ-SALGUERO, PEDRO |
分类号 |
C12N9/02;C12N15/53;C12Q1/68 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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