发明名称 |
METHOD OF SYNTHESIZING A FLUORESCENTLY LABELED PROTEIN |
摘要 |
The present invention provides a method of synthesizing a fluorescently labeled protein in a cell free protein synthesis system, comprising the steps of: (a) incubating a sample of ribosomes obtained from a cell-free extract with plasmid DNA containing a coding sequence for a protein of interest, said sample incubated in a coupled transcription/translation medium together with an aminoacyl tRNA having fluorescent label; (b) partially purifying said fluorescently labeled protein by separating newly synthesized, fluorescently-labeled protein from other fluorescent components within said sample; (c) measuring the amount of protein synthesized; (d) determining fluorescence of newly synthesized protein; and (e) determining the biological activity of the newly synthesized protein. |
申请公布号 |
CA2180176(A1) |
申请公布日期 |
1996.05.17 |
申请号 |
CA19952180176 |
申请日期 |
1995.11.02 |
申请人 |
RESEARCH DEVELOPMENT FOUNDATION |
发明人 |
KUDLICKI, WIESLAW;KRAMER, GISELA;HARDESTY, BOYD |
分类号 |
C12N15/09;C07K1/107;C12N9/10;C12P21/02;G01N33/533 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|