METHOD OF PREPARING OLIGONUCLEOTIDE PROBES OR PRIMERS, VECTOR THEREFOR AND USE THEREOF

摘要

A method of preparing a nucleic acid sequence capable of hybridizing to a target DNA sequence without requiring knowledge of this DNA sequence comprises the steps of ligating or otherwise linking a DNA fragment related to the target DNA sequence to a nucleotide sequence containing the recognition motif of an asymmetrically cleaving restriction enzyme, and subjecting the construct to the restriction enzyme to thereby cleave the construct within the targetrelated DNA fragment part thereof. A vector therefor comprises a site for insertion of the target-related DNA fragment, and a recognition sequence for an asymmetrically cleaving restriction enzyme or enzymes on one or both sides of said insertion site.