PRODUCTION OF RECOMBINANT PEPTIDES AS NATURAL HYDROPHOBIC PEPTIDE ANALORUES

摘要

A method for secreting a biologically active recombinant peptide as an analogue of a natural peptide having at least one hydrophobic region, by culturing cells transformed by a nucleic acid construct comprising elements for the expression and secretion of said peptide by the cell, and a sequence coding for a peptide with an amino acid sequence that differs from the natural peptide sequence in that it has at least one modification in a nontransmembrane hydrophobic region of the peptide; and by recovering the peptide and/or cells carrying said recombinant peptide. The resulting recombinant peptide, a corresponding DNA sequence, and a bacterium containing same, are also disclosed.