| 摘要 |
Recombinant antibodies or fragments thereof that are capable of binding to complexes formed between PNA (Peptide Nucleic Acid) and nucleic acids, particularly to PNA/DNA or PNA/RNA complexes, are described. The preferred antibodies are recombinant antibodies that bind to PNA/DNA OR PNA/RNA complexes, but not to single-stranded PNAs, double-stranded nucleic acids or single-stranded nucleic acids. PNAs are newly developed, not naturally occurring compounds of which some have a polyamide backbone bearing a plurality of ligands such as naturally occurring nucleobases attached to the backbone through a suitable linker. Some PNAs have been shown to possess a surprising high affinity for complementary nucleic acid, forming very stable and specific complexes. Such PNAs are thus suitable as hybridization probes for detection of nucleic acids. The antibodies now provided render the PNAs very usable as hybridization probes. The antibodies provided are useful in the capture, recognition, detection, identification or quantitation of nucleic acids in biological samples, via their ability to react with PNA-nucleic acid complexes. |
