PROCEDES DE DETECTION DU VIRUS DE L'IMMUNODEFICIENCE HUMAINE (VIH) FAISANT APPEL A DES AGENTS D'OXYDATION POUR AUGMENTER L'EXPRESSION VIRALE

摘要

An in vitro method for the detection of human immunodeficiency virus (HIV) in patient samples employing oxidative agents capable of augmenting viral expression is disclosed. Patient cells are prepared and an oxidative agent is added to the culture medium to induce HIV expression. The levels of intracellular and extracellular p24 are measured using conventional immunological assays. The number of HIV-infected patient cells can be quantitatively determined using the appropriate standards and controls.