HYBRID ADENOVIRUS-AAV VIRUS AND METHOD OF USE THEREOF
摘要
The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3' ITR sequences from an AAV, and a selected transgene. Also provided is a hybrid virus linked via a polycation conjugate to an AAV rep gene to form a single particle. These trans-infection particles are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors and viruses to produce large quantities of recombinant AAV.
申请公布号
WO9613598(A2)
申请公布日期
1996.05.09
申请号
WO1995US14018
申请日期
1995.10.27
申请人
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA;WILSON, JAMES, M.;KELLEY, WILLIAM, M.;FISHER, KRISHNA, J.
发明人
WILSON, JAMES, M.;KELLEY, WILLIAM, M.;FISHER, KRISHNA, J.