LONG LIFETIME ANISOTROPY (POLARIZATION) PROBES FOR CLINICAL CHEMISTRY, IMMUNOASSAYS, AFFINITY ASSAYS AND BIOMEDICAL RESEARCH

摘要

A method of conducting an immunoassay of a sample of interest is described, including the steps of: (A) coupling a luminescent asymmetric metal-ligand complex to the sample of interest to form a coupled sample; (B) exciting the coupled sample with linearly polarized electromagnetic energy to cause the coupled sample to emit fluorescent light; and (C) measuring the polarization of the fluorescent emission as a measure of a biological characteristic of the sample of interest. The measurement technique is schematically illustrated in the figure.