HYBRID ADENOVIRUS-AAV VIRUS AND METHOD OF USE THEREOF

摘要

The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5" and 3" ITR sequences from an AAV, and a selected transgene. Also provided is a hybrid virus linked via a polycation conjugate to an AAV rep gene to form a single particle. These trans-infection particles are characterized by higher titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors and viruses to produce large quantities of recombinant AAV.