改进的丙型肝炎诊断试剂

摘要

本发明涉及丙型肝炎病毒核心蛋白和非结构3蛋白(NS3)的抗原表位，包膜蛋白的抗原表位，非结构4蛋白的抗原表位，HCV非结构5蛋白的抗原表位以及含有这些表位的重组蛋白；生产重组蛋白的方法；一种包含重组蛋白的用于诊断待测血清样品中抗丙型肝炎病毒抗体的试剂；利用该试剂诊断丙型肝炎的方法。

主权项

1、一种丙型肝炎病毒的抗原蛋白，选自由KHCVCOREEPI，KHCU 518，KHCV NS4E，KHCV E1G，KHCVE2A，KHCV E2E和KHCV NS5-1.2组成的一组，其氨基酸序列如下：KHCV COREEPI：MetSerThrAsnProLysProGlnArgLysThrLysArgAsnThrAsnArgArgProGlnAspIleLysPheProGlyGlyGlyGlnIleValGlyGlyValTyrLeuLeuProArgArgGlyProArgLeuGlyValArgAlaThrArgLysThrSerGluArgSerGlnProArgGlyArgArgGlnProIleProLysAlaArgArgProGluGlyArgAlaTrpAlaGlnProGlyTyrProTrpProLeuTyrGlyAsnGluGlyLeuGlyTrpAlaGlyTrpLeuLeuSerProArgGly；KHCV 518：IleThrThrGlyAlaProIleThrTyrSerThrTyrGlyLysPheLeuAlaAspGlyGlyGlySerGlyGlyAlaTyrAspIleIleMetCysAspGluCysHisSerThrAspSerThrThrIleTyrGlyIleGlyThrValLeuAspGlnAlaGluThrAlaGlyAlaArgLeuValValLeuSerThrAlaThrProProGlySerValThrValProHisLeuAsnIleGluGluValAlaLeuSerAsnThrGlyGluIleProPheTyrGlyLysAlaIleProIleGluAlaIleLysGlyGlyArgHisLeuIlePheCysHisSerLysLysLysCysAspGluLeuAlaAlaLysLeuSerGlyLeuGlyLeuAsnAlaValAlaTyrTyrArgGlyLeuAspValSerValIleProThrSerGlyAspValValValValAlaThrAspAlaLeuMetThrGlyPheThrGlyAspPheAspSerValIleAspCysAsnThrCys；KHCV NS4E：IleIleProAspArgGluValLeuTyrGlnGluPheAspGluMetGluGluCysAlaSerHisLeuProTyrPheGluGlnGlyMetGlnLeuAlaGluGlnPheLysGlnLysAlaLeuGlyLeu；KHCV E1G：valSerGlnLeuPheThrPheSerProArgArgHisGluThrValGlnAspCysAsnCysSerIleTyrProGlyArgValSerGlyHisArgMetAlaTrpAspMetMetMetAsnTrpSerProThrThrAlaLeuValValSerGlnLeuLeuArgIleProGlnAlaValValAspMetValThrGlySerHisTrpGlyIleLeuAlaGlyLeuAlaTyrTyrSerMetValGlyAsnTrpAlaLysValLeuIleAlaMetLeuLeuPheAlaGlyValAspGlyThrThrHisValThrGly ；KHCV E2A：GlyAlaGlnGlyArgAlaAlaSerSerLeuThrSerLeuPheSerProGlyProValGlnHisLeuGlnLeuIleAsnThrAsnGlySerTrpHisIleAsnArgThrAlaLeuSerCysAsnAspSerLeuAsnThrGlyPheValAlaAlaLeuPheTyrLysTyrArgPheAsnAlaSerGlyCysProGluArgLeuAlaThrCysArgProIleAspThrPheAlaGlnGlyTrp；KHCV E2E：ThrArgGlygluArgCysAspLeuGluAspArgAspArgSerGluLeuSerProLeuLeuLeuSerThrThrGluTrpGlnValLeuProCysSerPheThrThrLeuProAlaLeuSerThrGlyLeuIleHlsLeuHisGlnAsnIlevalAspIleGlnTyrLeuTyrGlyIleGlySerAlaValValSerPheAlaIleLysTrpGluTyrIleValLeuLeuPheLeuLeuLeuAlaAspAla；andKHCV NS5-1.2：ThrGlyMetThrThrAspAsnValLysCysProCysGlnValProAlaProGluPhePheThrGluValAspGlyValArgLeuHisArgTyrAlaProAlaCysArgProLeuLeuArgGluGluValValPheGlnValGlyLeuHisGlnTyrLeuValGlySerGlnLeuProCysGluProGluProAspValAlaValLeuThrSerMetLeuThrAspProSerHisIleThrAlaGluThrAlaLysArgArgLeuAlaArgGlySerProProSerLeuAlaSerSerSerAlaSerGlnLeuSerAlaProSerLeuLysAlaThrCysThrThrHisHisAspSerProAspAlaAspLeuIleGluAlaAsnLeuLeuTrpArgGlnGluMetGlyGlyAsnIleThrArgValGluSerGluAsnLysValValIleLeuAspSerPheAspProLeuArgAlaGluAspAspGluGlyGluIleSerValProAlaGluIleLeuArgLysSerArgLysPheProProAlaLeuProIleTrpAlaProProAspTyrAsnProProLeuLeuGluSerTrpLysAspProAspTyrValProProValValHisGlyCysProLeuProProThrLysAlaProProIleProProProArgArgLysArgThrValValLeuThrGluSerThrValSerSerAlaLeuAlaGluLeuAlaThrLysThrPheGlySerSerGlySerSerAlaIleAspSerGlyThrAlaThrAlaProProAspGlnAlaSerGlyAspGlyAspArgGluSerAspValGluSerPheSerSerMetProProLeuGluGlyGluProGlyAspProAspLeuSerAspGlySerTrpSerThrValSerGluGluAlaSerGluAspValValCysCysSerMetSerTyrThrTrpThrGlyAlaLeuIleThrProCysAlaAlaGluGluSerLysLeuProIleAsnProLeuSerAsnSerLeuLeuArgHisHisAsnMetValTyrAlaThrThrSerArgSerAlaGlyLeuArgGlnLysLysValThrPheAspArgLeuGlnValLeuAspAspHisTyrArgAspValLeuLysGluMetLysAlaLysAla.