| 摘要 |
PURPOSE: To make it possible to analyze L type and D type amino acid effectively by conducting capillary electrophoresis using specified carrier buffer solution containing phosphate buffer solution or the like. CONSTITUTION: Carrier buffer solution contains 30 to 100mM of phosphate buffer solution, 50 to 150mM of β-cyclodextrin, 3 to 6M of urea, and 50 to 100mM of sodium dodecyl sulfate (SDS), and its pH is set to 6.0 to 8.0. The conditions of capillary electrophoresis are, for example, detection wavelength of 185 to 220nm, applied voltage of 8 to 30kV, and capillary effective length of 75 to 100μm×30 to 100cm, and sample injection is performed by a weighing method (for 60 seconds). In a CE system portion S, both ends of a hollow capillary 1 are soaked in jars of carrier buffer solution 2, the inside of the capillary 1 is filled fully with the carrier buffer solution 2, and electrodes 3 are connected to both end jars of the carrier buffer solution 2 with a high voltage power supply V so as to detect by a detector 4. |
