发明名称 |
USE OF SPERMIDINE TO RELIEVE INHIBITION OF LIGASE CHAIN REACTION IN A CLINICAL TEST SAMPLE |
摘要 |
An improved method of amplifying target nucleic acids and particularly, to a method of alleviating the inhibitory effect of certain samples on ligase chain reaction amplifications. The relief of inhibition is accomplished by adding to the amplification mixture a polyamine reagent such as spermidine, in effective amounts, typically 1 to 4 nM. In other embodiments, the presence of spermidine allows for amplification to proceed in the presence of reduced concentrations of MgCl2 and ligase.
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申请公布号 |
CA2202990(A1) |
申请公布日期 |
1996.05.02 |
申请号 |
CA19952202990 |
申请日期 |
1995.10.18 |
申请人 |
ABBOTT LABORATORIES;ABBOTT LABORATORIES |
发明人 |
DAVIS, ALAN H.;LEE, EUI H.;CAO, JIANLI |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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